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CSMD1���w,Anti-CSMD1���w

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�ͷ��Ԓ��021 60340850    021 60340830    021 �ͷ�QQ ��827774711    349199432     372584443 �a(ch��n)Ʒ���Q  CSMD1���w,Anti-CSMD1���w                                              �a(ch��n)Ʒ��̖(h��o) BYk-1579R    

���P(gu��n)��(bi��o)ӛ HRP Biotin Gold RBITC AP FITC Cy3 Cy5 Cy5.5 Cy7 PE PE-Cy3 PE-Cy5 PE-Cy5.5 PE-CY7 APC Alexa Fluor 350 Alexa Fluor 488 Alexa Fluor 555 Alexa Fluor 647 �� �� 1mg/1ml ���w��Դ Rabbit OR MOUSE��¡��� polyclonal or monoclonal�a(ch��n)Ʒ��� һ���a(ch��n)Ʒ��;  ���Ќ�(sh��)�(y��n)���������߽M����(sh��)�(y��n)��WB��(sh��)�(y��n)��IF��IP��ELISA��(sh��)�(y��n)������(y��ng)�Ę�(bi��o)ӛ���w��HRP��(bi��o)ӛ���w��F(xi��n)ITC��(bi��o)ӛ��BIO�ȡ� �� �� Lyophilized or Liquid �� �� IgG     

CSMD1���w,Anti-CSMD1���w����l�� Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month ��nd for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

CSMD1���w,Anti-CSMD1���w�a(ch��n)Ʒ�f����

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ԓ��˾�a(ch��n)Ʒ��� ȮELISAԇ���� �uELISAԇ���� �iELISAԇ���� ����ELISAԇ���� ؈ELISAԇ���� �RELISAԇ���� ֲ��ELISAԇ���� ����ELISAԇ���� ɽ��ELISAԇ���� �~ELISAԇ���� ��ELISAԇ���� ֲ��ELISAԇ���� ����ELISAԇ���� �~ELISAԇ���� ��ELISAԇ���� ����������������������ԇ���� �Ҹ����(xi��ng)���������� �Ҹ����(xi��ng)����Ҏ(gu��)���� ����� ��(x��)�������

��26S����ø�w��ATPø�{(di��o)��(ji��)����11(PSMD11) ELISA kit؛̖(h��o):SY-E17857h

��26S����ø�w��ATPø�{(di��o)��(ji��)����11��PSMD11�� ELISA kit  Ӣ�����Q�� Human proteasome ��prosome, macropain�� 26S subunit, non-ATPase, 11 ��PSMD11�� ELISA kit  �e���� MGC3844, Rpn6, S9, p44.5, 26S proteasome regulatory subunit 9|proteasome 26S non-ATPase subunit 11  ؛̖(h��o)�� CSB-E17857h  ���ăr(ji��)�� 3600  Ҏ(gu��)�� 96T  �N�٣� Human  ���y(c��)�����Q�� proteasome ��prosome, macropain�� 26S subunit, non-ATPase, 11  �s���� PSMD11  �z�y(c��)������ Request Information  �`���ȣ� Request Information  ����(y��ng)�r(sh��)�g�� 1-5h  ����ӱ��w�e�� 50-100ul  �z�y(c��)���L�� 450 nm  ���d�� ע:�a(ch��n)Ʒ�f���������и��M(j��n)��Ո(q��ng)ֱ���cCUSABIO�a(ch��n)Ʒ���T“(li��n)ϵ����ȡ���°��f������  ��;�� For research use only. Not for diagnostic use.  ԭ�� �� This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PSMD11 has been pre-coated onto a microplate. Standards ��nd samples are pipetted into the wells ��nd any PSMD11 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PSMD11 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase ��HRP�� is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells ��nd color develops in proportion to the amount of PSMD11 bound in the initial step. The color development is stopped ��nd the intensity of the color is measured.  �خ��ԣ� This assay has high sensitivity ��nd excellent specificity for detection of Human PSMD11. No signifiCANt cross-reactivity or interference between Human PSMD11 ��nd analogues was observed.  ���ܶȣ� Intra-assay Precision ��Precision within an assay��: CV%<8%Three samples of known concentration were tested twenty times on one plate to assess.Inter-assay Precision ��Precision between assays��: CV%<10%Three samples of known concentration were tested in twenty assays to assess.  �ӱ��Ѽ�����(ch��)�棺 Serum: Use a serum separator tube ��SST�� ��nd allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 15 minutes at 1000 ×g. Remove serum ��nd assay immediately or aliquot ��nd store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles.Plasma: Collect plasma using EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 ×g at 2-8°C within 30 minutes of collection. Assay immediately or aliquot ��nd store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles.  �z�y(c��)���E�� Bring all reagents ��nd samples to room temperature before use. Centrifuge the sample again after thawing before the assay. It is recommended that all samples ��nd standards be assayed in duplicate.1. Prepare all reagents, working standards, ��nd samples as directed in the previous sections.2. Refer to the Assay Layout Sheet to determine the number of wells to be used ��nd put any remaining wells ��nd the desiccant back into the pouch ��nd seal the ziploc, store unused wells at 4°C.3. Add 100μl of standard ��nd sample per well. Cover with the adhesive strip provided. Incubate for 2 hours at 37°C. A plate layout is provided to record standards ��nd samples assayed.4. Remove the liquid of each well, don��t wash.5. Add 100μl of Biotin-antibody ��1x�� to each well. Cover with a new adhesive strip. Incubate for 1 hour at 37°C. ��Biotin-antibody ��1x�� may appear cloudy. Warm up to room temperature ��nd mix gently until solution appears uniform.��6. Aspirate each well ��nd wash, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer ��200μl�� using a squirt bottle, multi-channel pipette, manifold dispenser, or autowasher, ��nd let it stand for 2 minutes, complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining wash Buffer by aspirating ordecanting. Invert the plate ��nd blot it against clean paper towels.7. Add 100μl of HRP-avidin ��1x�� to each well. Cover the microtiter plate with a new adhesive strip. Incubate for 1 hour at 37°C.8. Repeat the aspiration/wash process for five times as in step 6.9. Add 90μl of TMB Substrate to each well. Incubate for 15-30 minutes at 37°C. Protect from light.10. Add 50μl of Stop Solution to each well, gently tap the plate to ensure thorough mixing.11. Determine the optical density of each well within 5 minutes, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. Subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher ��nd less accurate.  �Y(ji��)��Ӌ(j��)�㣺 Using the professional soft "Curve Expert 1.3" to make a standard curve is recommended, which can be downloaded from our web.Average the duplicate readings for each standard ��nd sample ��nd subtract the average zero standard optical density.Create a standard curve by reducing the data using computer software capable of generating a four parameter logistic ��4-PL�� curve-fit. As an alternative, construct a standard curve by plotting the mean absorbance for each standard on the x-axis against the concentration on the y-axis ��nd draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the PSMD11 concentrations versus the log of the O.D. ��nd the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data.If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor. 

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MSMD042P1UMSMD042P1U

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750W���БT������ͨ����:MDMA082P1G+MDDDT3530ͨ����MDMA082P1G+MDDDT3530003�}�_��750W���БT�������x܇:ͨ���ͣ�MDMA082P1H+MDDDT3530ͨ���� MDMA082P1H+MDDDT3530003�}�_��1000W��С�T����MSMA102P1G+MDDDT35301000W ���БT������ͨ����MDMA102P1G+MDDDT3530  �}�_�ͣ�MDMA102P1G+MDDDT3530003���x܇��MDMA102P1H+MDDDT3530ͨ����       MDMA102P1H+MDDDT3530003�}�_��1000w����T����MHMA102P1G+MDDDT3530ͨ��MHMA102P1G+MDDDT3530003�}�_��MSMA102P1H  MDMA102P1H  MHMA102P1H

 MSMA152P1G  MDMA152P1G1500W����T����:ͨ����MHMA152P1G+MDDDT5540 �}�_�ͣ�MHMA152P1G+MDDDT5540003MSMA152P1H  MDMA152P1H  MHMA152P1H

MSMA202P1G   MHMA202P1G2000W���БT����ͨ����MDMA202P1G+MEDDT7364 MDMA202P1G+MEDDT7364003 MSMA202P1H   MDMA202P1H  MHMA202P1GH 

3000W��С�T���� ͨ���ͣ�MSMA302P1G+MFDDTA390�}�_�ͣ�MSMA302P1G+MFDDTA3900033000W���БT������ͨ����:MDMA302P1G+MFDDTA390�}�_�ͣ�MDMA302P1G+MFDDTA3900033000W����T������ͨ���ͣ�MHMA302P1G+MFDDTA390�}�_�ͣ�MHMA302P1G+MFDDTA3900033000W����T�����x܇����ͨ���ͣ�MHMA302P1H+MFDDTA390�}�_�ͣ�MHMA302P1H+MFDDTA3900033000W��С�T�����x܇����ͨ���ͣ�MSMA302P1H+MFDDTA390�}�_�ͣ�MSMA302P1H+MFDDTA3900033000W���БT�����x܇����ͨ���ͣ�MDMA302P1H+MFDDTA390�}�_�ͣ�MDMA302P1H+MFDDTA3900034000W���БT����MDMA402P1G+ MFDDTB3A2ͨ����MDMA402P1G+MFDDTB3A2003�}�_��4000W����T����MHMA402P1G+MFDDTB3A2 ͨ����MHMA402P1G+MFDDTB3A2003�}�_��4000W��С�T����MSMA402P1G+MFDDTB3A2ͨ����MSMA402P1G+MFDDTB3A2003�}�_��4000W��С�T����MSMA402P1H+MFDDTB3A2ͨ����MSMA402P1H+MFDDTB3A2003�}�_��  4000W���БT����MDMA402P1H+MFDDTB3A2ͨ����MSMA402P1H+MFDDTB3A2003�}�_��

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